In addition , the heparinase is stimulated in the presence of ca2 + and mn2 + , but is inhibited by cu2 + and fe3 + . neither pmsf nor edta significantly affected enzyme activity . by infrared spectrum and proton resonance spectrum analysis , we found smaller polysaccharides , unsaturated bond and reducing sugar , which are specific products by heparinase 在產(chǎn)物分析中發(fā)現(xiàn),該酶作用于肝素后,還原糖數(shù)量明顯增加,通過紅外色譜及質子譜的分析發(fā)現(xiàn),產(chǎn)物中出現(xiàn)了含不飽和雙鍵的還原寡糖,這是肝素酶作用于肝素后的特異性產(chǎn)物,從而可以進一步證明該菌確實產(chǎn)生肝素酶。